Fluorescence studies on anthryl bacteriorhodopsins

Singh, Anil K. ; Roy, Mita (1991) Fluorescence studies on anthryl bacteriorhodopsins Journal of Photochemistry and Photobiology B: Biology, 8 (3). pp. 325-335. ISSN 1011-1344

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Official URL: http://www.sciencedirect.com/science/article/pii/1...

Related URL: http://dx.doi.org/10.1016/1011-1344(91)80089-Z


Fluorescence properties of bacteriorhodopsin analogues containing 3-methyl-5-(9'-anthryl)-2E,4E-pentadienal and 3,7-dimethyl-9-(9'-anthryl)-2E,4E,6E,8E-nonatetraenal chromophores as built-in fluorescence probes are described. Determination of fluorescence quantum yields, fluorescence quenching and energy transfer efficiencies in terms of the Förster's energy transfer between tryptophans (Trp) and the anthrylidene moiety indicates that Trp residues lie at a critical distance (R0) of ca. 27 Å from the chromophore bound at Lys-216 on the apo-protein.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Bacteriorhodopsin; Bacteriorhodopsin Analogues; Fluorescence; Energy Transfer
ID Code:54093
Deposited On:11 Aug 2011 11:45
Last Modified:11 Aug 2011 11:45

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