Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latifolia Roxb.)

Sankara Rao, K. (1986) Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latifolia Roxb.) Plant Cell Reports, 5 (3). pp. 199-201. ISSN 0721-7714

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Official URL: http://www.springerlink.com/content/qr3j1645u64073...

Related URL: http://dx.doi.org/10.1007/BF00269118

Abstract

Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.

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Deposited On:11 Aug 2011 12:05
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