Mycobacterium tuberculosis pantothenate kinase: possible changes in location of ligands during enzyme action

Chetnani, B. ; Das, S. ; Kumar, P. ; Surolia, A. ; Vijayan, M. (2009) Mycobacterium tuberculosis pantothenate kinase: possible changes in location of ligands during enzyme action Acta Crystallographica Section D, 65 . pp. 312-325. ISSN 0907-4449

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Official URL: http://scripts.iucr.org/cgi-bin/paper?be5119

Related URL: http://dx.doi.org/10.1107/S0907444909002170

Abstract

The crystal structures of complexes of Mycobacterium tuberculosis pantothenate kinase with the following ligands have been determined: (i) citrate; (ii) the nonhydrolysable ATP analogue AMPPCP and pantothenate (the initiation complex); (iii) ADP and phosphopantothenate resulting from phosphorylation of pantothenate by ATP in the crystal (the end complex); (iv) ATP and ADP, each with half occupancy, resulting from a quick soak of crystals in ATP (the intermediate complex); (v) CoA; (vi) ADP prepared by soaking and cocrystallization, which turned out to have identical structures, and (vii) ADP and pantothenate. Solution studies on CoA binding and catalytic activity have also been carried out. Unlike in the case of the homologous Escherichia coli enzyme, AMPPCP and ADP occupy different, though overlapping, locations in the respective complexes; the same is true of pantothenate in the initiation complex and phosphopantothenate in the end complex. The binding site of MtPanK is substantially preformed, while that of EcPanK exhibits considerable plasticity. The difference in the behaviour of the E. coli and M. tuberculosis enzymes could be explained in terms of changes in local structure resulting from substitutions. It is unusual for two homologous enzymes to exhibit such striking differences in action. Therefore, the results have to be treated with caution. However, the changes in the locations of ligands exhibited by M. tuberculosis pantothenate kinase are remarkable and novel.

Item Type:Article
Source:Copyright of this article belongs to International Union of Crystallography.
Keywords:Pantothenate Kinase; Mycobacterium tuberculosis; Ligand Location
ID Code:53847
Deposited On:10 Aug 2011 09:39
Last Modified:21 Dec 2011 06:40

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