F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through LeX-LeX interaction

Kumar, Mukesh ; Sarkar, Debi P. (1996) F protein induced fusion of Sendai viral envelopes with mouse teratocarcinoma cells through LeX-LeX interaction FEBS Letters, 391 (1-2). pp. 17-20. ISSN 0014-5793

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0014-5793(96)00698-9

Abstract

The efficiency of membrane fusion between reconstituted Sendau viral envelopes containing only the fusion protein (F-virosomes) and the plasma membrane of mouse teratocarcinoma cells (F9) in culture was assessed using an assay based on the relief of self-quenching of a lipid probe incorporated in the F-virosomes. The potential of F-virosomes was also evaluated for a targeted cytosolic delivery of lysozyme to F9 cells. [125I]Lysozyme entrapped into F-virosomes was taken to examine its fusion-mediated transfer to the F9 cells. Target specificity of the F-virosomes was confirmed by the interaction between the terminal Lex moiety (Ga1β1 → 4(Fucα1 → 3)GlcNac) of F protein and the Lex determinant on the membrane of F9 cells. Incubation of the loaded F-virosomes with cells led to fusion-mediated delivery, as inferred from the ability of cells to internalize lysozyme in the presence of azide (a potent inhibitor of endocytosis). These results suggest that carbohydrate-carbohydrate interaction is strong enough for target cell recognition followed by phospholipid bilayer melding induced by fusion glycoprotein of Sendai virus.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Sendai; Virosome; Lex/CD15; Membrane Fusion
ID Code:53408
Deposited On:08 Aug 2011 12:17
Last Modified:08 Aug 2011 12:17

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