Differences in dispersion of influenza virus lipids and proteins during fusion

Lowy, R. Joel ; Sarkar, Debi P. ; Whitnall, Mark H. ; Blumenthal, Robert (1995) Differences in dispersion of influenza virus lipids and proteins during fusion Experimental Cell Research, 216 (2). pp. 411-421. ISSN 0014-4827

Full text not available from this repository.

Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1006/excr.1995.1052


Digitally enhanced low-light-level fluorescence video microscopy and immunochemical staining were used to examine influenza virus envelope lipid and protein redistribution during pH-induced fusion. Video microscopy was performed using viruses labeled with either the lipid analogue octadecylrhodamine B (R18) or fluorescein isothiocyanate (FITC) covalently linked to envelope proteins. Viruses were bound to human red blood cells, and the pattern and intensity of fluorescence were monitored for 30 min while cell-virus complexes were perfused with pH 7.4 or 4.8 media at temperatures either above or below 20°C. R18 showed complete redistribution and dequenching by 30 min at all incubation temperatures, confirming reports that viral fusion occurs at subphysiological temperatures. FITC-labeled protein showed spatial redistribution at 28°C but no change at low temperature. Electron microscopy observations of immunochemical staining of viral proteins confirmed both that protein redistribution at 37°C was slower than R18 and the failure of movement within 30 min at 16°C. Video microscopy monitoring of RNA staining by acridine orange of virus-cell complexes showed redistribution to the RBCs at all temperatures but only after low pH-induced fusion. The results are consistent with differential dispersion of viral components into the RBC and the existence of relatively long-lived barriers to diffusion subsequent to fusion pore formation.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:53407
Deposited On:08 Aug 2011 12:17
Last Modified:10 Aug 2011 07:07

Repository Staff Only: item control page