Saravanan, Settu ; Raghava Rao, Jonnalagadda ; Nair, Balachandran Unni ; Ramasami, Thirumalachari (2008) Aqueous two-phase poly(ethylene glycol)-poly(acrylic acid) system for protein partitioning: influence of molecular weight, pH and temperature Process Biochemistry, 43 (9). pp. 905-911. ISSN 0032-9592
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Official URL: http://www.sciencedirect.com/science/article/pii/S...
Related URL: http://dx.doi.org/10.1016/j.procbio.2008.04.011
Abstract
The partitioning behavior of two model proteins in poly(ethylene glycol) (PEG)-poly(acrylic acid) (PAA) aqueous two-phase systems has been studied. The effect of polymer molecular weight, tie line length (TLL), pH, temperature and NaCl concentration on the partitioning of proteins (ovalbumin and myoglobin) of different molecular weights and isoelectric points has been studied. Poly(ethylene glycol) is enriched in the top phase while poly(acrylic acid) is found in the bottom phase. An increased partitioning of proteins to the PEG phase has been observed with increased TLL. The protein partitioning decreases on increasing the molecular weight of PEG and temperature, whereas the partitioning increases with increase in pH and NaCl concentration of the system. The protein partitioning is better for both the model proteins using PEG 4000-PAA systems at 20 °C, pH 8.0 with 1 M NaCl. The percentage yield of extraction for myoglobin and ovalbumin is 95.2% and 87.4%, respectively.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Aqueous Two-phase Systems; Globular Proteins; Poly(ethylene Glycol); Poly(acrylic Acid); Ovalbumin; Myoglobin; Partition Coefficient |
| ID Code: | 53346 |
| Deposited On: | 10 Aug 2011 09:57 |
| Last Modified: | 10 Aug 2011 09:57 |
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