Chromosome doubling of barley haploids by nitrous oxide and colchicine treatments

Subrahmanyam, N. C. ; Kasha, K. J. (1975) Chromosome doubling of barley haploids by nitrous oxide and colchicine treatments Canadian Journal of Genetics and Cytology, 17 (4). pp. 573-583. ISSN 0008-4093

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Hybridization of Hordeum vulgare L. (2n = 14) with H. bulbosum L. (2n = 14) results in a high frequency of haploids of H. vulgare through selective elimination of H. bulbosum chromosomes. Doubled haploids were produced by nitrous oxide (N2O) or 0.1% colchicine with and without dimethyl sulfoxide (DMSO) treatments. Pollinated florets (vulgare × bulbosum) were treated with N2O under 21.1 × 10-3 to 42.2 × 10-3 kg/m2 pressure (30 to 60 psi) and the frequency of doubled haploids ranged from 0 to 100%, depending upon pressure and duration of exposure. However, the frequency of seedlings from the most effective N2O doubling treatment was very low (0.5%) whereas 17% of the florets pollinated in the controls resulted in seedlings. Of the haploid seedlings treated with either 0.1% colchicine or colchicine plus DMSO, doubled sectors occurred in 37.4 and 55.8% respectively. Seed was also obtained from 3% of the untreated plants indicating a low frequency of natural chromosome doubling. The proportion of doubled tillers per plant was 61.6% in colchicine plus DMSO treatments and 30.8% in the colchicine treatments. Colchicine plus DMSO was the most efficient treatment for doubling barley haploids during early stages of development.

Item Type:Article
Source:Copyright of this article belongs to NRC Research Press.
ID Code:53161
Deposited On:05 Aug 2011 07:22
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