Isolation of a cdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle

Ghosh, Santanu Kumar ; Sinha, Pratima (2000) Isolation of a cdc28 mutation that abrogates the dependence of S phase on completion of M phase of the budding yeast cell cycle Journal of Genetics, 79 (3). pp. 91-96. ISSN 0022-1333

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Official URL: http://www.ias.ac.in/jgenet/Vol79No3/JG478.PDF

Related URL: http://dx.doi.org/10.1007/BF02715856

Abstract

We have isolated a mutation in the budding yeast Saccharomyces cerevisisae CDC28 gene that allows cdc13 cells, carrying damaged DNA, to continue with the cell division cycle. While cdc13 mutant cells are arrested as largebudded cells at the nonpermissive temperature 37°C, the cdc13 cdc28 double mutant culture showed cells with one or more buds, most of which showed apical growth. The additional buds emerged without the intervening steps of nuclear division and cell separation. We suggest that the cdc28 mutation abrogates a checkpoint function and allows cells with damaged or incompletely replicated DNA an entry to another round of cell cycle and bypasses the mitotic phase of the cell cycle.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Yeast; Cdc28 Kinase; Checkpoint; Cell Cycle
ID Code:52899
Deposited On:04 Aug 2011 12:18
Last Modified:18 May 2016 06:16

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