Controlled delivery of diphtheria toxoid using biodegradable poly(D, L-lactide) microcapsules

Singh, Manmohan ; Singh, Amarjit ; Talwar, Gursharan Prasad (1991) Controlled delivery of diphtheria toxoid using biodegradable poly(D, L-lactide) microcapsules Pharmaceutical Research, 8 (7). pp. 958-961. ISSN 0724-8741

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Official URL: http://www.springerlink.com/content/m20885510mwk28...

Related URL: http://dx.doi.org/10.1023/A:1015832302605

Abstract

Diphtheria toxoid, which is an important vaccine in the expanded program of immunization (EPI) in the developing countries, was microencapsulated using poly(D, L,-lactide) of 49,000 molecular weight and the in-water drying technique. The microcapsules were subjected to an in vitro antigen release study using a sensitive enzyme-linked immunosorbent assay (ELISA) developed in the laboratory. Antibody titers in immunized Balb/C mice were also determined using direct ELISA. The antibody units in the immunized group till day 75 were quite comparable to those in the group receiving conventional three-dose injection of diphtheria toxoid with calcium phosphate as an adjuvant. SEM photographs of the micro-capsules during in vitro degradation demonstrated the erosion kinetics of the polymer, leading to controlled release of the antigen.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Vaccine Delivery; Biodegradable Microcapsules; Antigen ELISA; Erosion Kinetics; Antibody Titers
ID Code:52874
Deposited On:04 Aug 2011 09:50
Last Modified:12 Aug 2011 08:40

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