Enzyme synthesis following conjugation and recombination in Escherichia coli

Joshi, G. P. ; Siddiqi, O. (1968) Enzyme synthesis following conjugation and recombination in Escherichia coli Journal of Molecular Biology, 32 (2). pp. 201-210. ISSN 0022-2836

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0022-2836(68)90004-1


The structural gene for alkaline phosphatase, when injected by an Hfr into a constitutive recipient, does not function extensively. After an initial spurt, phosphatase synthesis suffers an eclipse and is not resumed until the P+ gene is integrated into the recipient's chromosome. The time-course of genetic recombination was investigated by following the synthesis of alkaline phosphatase in crosses between allelic phosphatase-negative mutants. The appearance of PP+ recombinant nuclei in such crosses is paralleled by an increase in the differential rate of enzyme synthesis. Functional recombinants arise 30 to 40 minutes after the entry of the Hfr P gene and multiply exponentially from the time they originate. The kinetics of recombination are unrelated to the generation time of the F. In a recipient carrying a thermosensitive mutation which blocks DNA replication at 42°C, recombination occurs in the absence of DNA replication. Interference with DNA replication, in fact, greatly enhances the rate of recombination.

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ID Code:52786
Deposited On:04 Aug 2011 08:35
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