DNA synthesis during bacterial conjugation: I. Effect of mating on DNA replication in Escherichia coli Hfr

Vijay Sarathy, P. ; Siddiqi, O. (1973) DNA synthesis during bacterial conjugation: I. Effect of mating on DNA replication in Escherichia coli Hfr Journal of Molecular Biology, 78 (3). pp. 427-441. ISSN 0022-2836

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0022-2836(73)90466-X

Abstract

Conjugation in Escherichia coli involves an oriented transfer of DNA from the Hfr to the F. We have examined the course of DNA replication in a donor cell while it is transferring its DNA. Using isotopic density shift for estimating replication, we have shown that mating is accompanied by initiation of a new round of DNA replication in Hfr cells. With the onset of F-mediated transfer replication, the normal vegetative replication in the Hfr appears to be suppressed. Experiments with F' donors indicate that the transfer of the chromosome is necessary for switching off vegetative replication.

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