Integration of donor DNA in bacterial conjugation

Siddiqi, O. ; Fox, M. S. (1973) Integration of donor DNA in bacterial conjugation Journal of Molecular Biology, 77 (1). pp. 101-123. ISSN 0022-2836

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Conjugation between 13C15N- and 3H-labelled hybrid donors and 13C15N-labelled hybrid recipients of Escherichia coli gives rise to recombinant radioactive DNA of density greater than labelled hybrid. The donor radioactivity is present, in these molecules, in discrete heavy segments covalently attached to the light strand. When light radioactive Hfr cells are mated to heavy F cells in light medium, the donor label appears, in DNA extracted from the F cells, in labelled hybrid molecules. The radioactivity in these molecules is exclusively in the light strand. The insertion of donor material is thus restricted to a single newly formed strand of the recipient DNA and double-strand integrations do not occur. A temperature-sensitive recipient containing the dna B mutation ts43 accumulates single-stranded Hfr DNA if mating is carried out at the nonpermissive temperature. The formation of a complementary strand in the recipient does not, therefore, appear to be necessary for continued transfer of Hfr DNA.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:52768
Deposited On:04 Aug 2011 08:36
Last Modified:04 Aug 2011 08:36

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