Structural transformations in protein crystals caused by controlled dehydration

Salunke, D. M. ; Veerapandian, B. ; Kodandapani, R. ; Vijayan, M. (1985) Structural transformations in protein crystals caused by controlled dehydration Journal of Biosciences, 8 (1-2). pp. 37-44. ISSN 0250-5991

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Recent experiments in this laboratory on structural transformations caused by controlled dehydration of protein crystals have been reviewed. X-ray diffraction patterns of the following crystals have been examined under varying conditions of environmental humidity in the relative humidity range of 100-75%: a new crystal form of bovine pancreatic ribonuclease A grown from acetone solution in tris buffer (I), the well-known monoclinic form of the protein grown from aqueous ethanol (II), the same form grown from a solution of 2-methyl pentan-2,4-diol in phosphate buffer (III), tetragonal (IV), orthorhombic (V), monoclinic (VI) and triclinic (VII) hen egg white lysozyme, porcine 2 Zn insulin (VIII), porcine 4 Zn insulin (IX) and the crystals of concanavalin A(X). I, II, IV, V and VI undergo one or more transformations as evidenced by discontinuous changes in the unit cell dimensions, the diffraction pattern and the solvent content. Such water-mediated transformations do not appear to occur in the remaining crystals in the relative humidity range explored. The relative humidity at which the transformation occurs is reduced when 2-methyl pentan-2,4-diol is present in the mother liquor. The transformations are affected by the crystal structure but not by the amount of solvent in the crystals. The X-ray investigations reviewed here and other related investigations emphasize the probable importance of water-mediated transformations in exploring hydration of proteins and conformational transitions in them.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Water-mediated Transformations; Environmental Humidity; Protein Crystals; Conformational Transitions; Protein Hydration
ID Code:52576
Deposited On:04 Aug 2011 07:47
Last Modified:18 May 2016 06:04

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