Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae)

Sulaiman, I. M. ; Rangaswamy, N. S. ; Babu, C. R. (1991) Formation of plantlets through somatic embryogeny in the Himalayan blue poppy, Meconopsis simplicifolia (Papaveraceae) Plant Cell Reports, 9 (10). pp. 582-585. ISSN 0721-7714

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Official URL: http://www.springerlink.com/content/n7272222477n24...

Related URL: http://dx.doi.org/10.1007/BF00232337

Abstract

Exuberant and subculturable calli could be induced from only hypocotyl and leaf segments of ca 4-month-old seedlings of Meconopsis simplicifolia cultured on Murashige & Skoog's medium supplemented with 10-6M kinetin + 10≄5M α -naphthalene acetic acid. Suspension cultures were initiated from the calli in a similar medium but with 10≄5Mα 2,4-dichlorophenoxy acetic acid in place ofα-naphthalene acetic acid. In ca 80% of the suspension cultures somatic embryos differentiated freely (80-85%) as well as on the surface of small clumps of tissue (15-20%). Somatic embryos that developed beyond heart-shaped stage were transferred to agar-solidified Murashige & Skoog's medium free of growth substances. When maintained in 10 h light and 14 h dark the somatic embryos developed into plantlets bearing cauline leaves. From seed sowing to raising normal plantlets via callus required 28 weeks; on average 80 plantlets were obtained from one explant in three passages.

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