Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars

Kumar, S. ; Srivastava, A. ; Raj, S. K. (2005) Molecular detection of Cucumber mosaic virus in Chrysanthemum cultivars Acta Phytopathologica et Entomologica Hungarica, 40 (3-4). pp. 205-211. ISSN 0238-1249

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Molecular detection of Cucumber mosaic virus in various chrysanthemum cultivars was attempted by RT-PCR and Southern hybridization tests using CMV specific primers and cloned probes, respectively. A pair of primers was designed from a conserved region of the Cucumber mosaic virus coat protein (CMV-CP) gene, capable of amplifying a product of ~650 bp from various CMV strains. RT-PCR using the total nucleic acid from infected leaf samples and the specific primers resulted in positive amplification of an expected size band of ~650 bp in most of the samples. The identity of the PCR amplicons was checked by Southern hybridization using the a32P-labelled DNA probes prepared from the cloned coat protein gene of a well-identified strain of CMV isolated from Amaranthus. Positive signal of hybridization of PCR products and CMV probes confirmed the identity of fected chrysanthemum samples.

Item Type:Article
Source:Copyright of this article belongs to Akadémiai Kiadó.
Keywords:Molecular Detection of Cucumber Mosaic Virus; Molecular Detection of Cucumber Mosaic Virus
ID Code:52176
Deposited On:02 Aug 2011 07:58
Last Modified:02 Aug 2011 07:58

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