Achwal, Chhaya W. ; Iyer, Chitra A. ; Sharat Chandra, H. (1983) Immunochemical evidence for the presence of 5mC, 6mA and 7mG in human, Drosophila and mealybug DNA FEBS Letters, 158 (2). pp. 353-358. ISSN 0014-5793
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001457...
Related URL: http://dx.doi.org/10.1016/0014-5793(83)8
Abstract
We have reported that production and characterization of antibodies highly specific to 5-methyl-cytosine (5mC) and the development of a sensitive immunochemical method for the detection of 5mC in DNA. Extension of this method to two other modified bases, 6-methyladenine (6mA) and 7-methylguanine (7mG), is reported here. By use of this immunochemical approach, we are able to detect 5mC, 6mA and 7mG in human and Drosophila DNA and confirm their presence in the DNA of two mealybug species.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Federation of European Biochemical Societies. |
| Keywords: | DNA Methylation; Biotin-avidin Cross-linking; DNA-protein Interaction; Gene Regulation Z-DNA; X-chromosome Inactivation |
| ID Code: | 5180 |
| Deposited On: | 18 Oct 2010 06:35 |
| Last Modified: | 16 May 2016 15:43 |
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