Type III DNA restriction and modification systems EcoP1 and EcoP15: nucleotide sequence of the EcoP1 operon, the EcoP15mod gene and some EcoP1mod mutants

Hümbelin, Markus ; Suri, Bruno ; Rao, Desirazu N. ; Hornby, David P. ; Eberleshort, Helen ; Pripfl, Theres ; Kenel, Susanne ; Bickle, Thomas A. (1988) Type III DNA restriction and modification systems EcoP1 and EcoP15: nucleotide sequence of the EcoP1 operon, the EcoP15mod gene and some EcoP1mod mutants Journal of Molecular Biology, 200 (1). pp. 23-29. ISSN 0022-2836

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/002228...

Related URL: http://dx.doi.org/10.1016/0022-2836(88)90330-0

Abstract

This paper presents the nucleotide sequence of the mod-res operon of phage P1, which encodes the two structural genes for the EcoP1 type III restriction and modification system. We have also sequenced the mod gene of the allelic EcoP15 system. The mod gene product is responsible for binding the system-specific DNA recognition sequences in both restriction and modification; it also catalyses the modification reaction. A comparison of the two mod gene product sequences shows that they have conserved amino and carboxyl ends but have completely different sequences in the middle of the molecules. Two alleles of the EcoP1mod gene that are defective in modification but not in restriction were also sequenced. The mutations in both alleles lie within the non-conserved regions.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:AdoMet; S-adenosylmethionine
ID Code:51220
Deposited On:28 Jul 2011 07:23
Last Modified:28 Jul 2011 07:23

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