Properties and characteristics of an anti-human chorionic gonadotropin monoclonal antibody

Gupta, S. K. ; Ramakrishnan, S. ; Talwar, G. P. (1982) Properties and characteristics of an anti-human chorionic gonadotropin monoclonal antibody Journal of Biosciences, 4 (1). pp. 105-114. ISSN 0250-5991

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The product of a hybrid cell clone, P3W80, obtained as ascites fluid from mouse peritoneal cavity had high titres of anti-human chorionic gonadotropin antibodies e.g. 30 to 40% binding of 125I-human chorionic gonadotropin at 107 dilution in a radioimmunoassay. The antiserum SB6 (raised against β-human chorionic gonadotropin distributed by National Institutes of Health, USA gave similar binding at 5000 dilution in parallel runs. The monoclonal antibody recognized best human chorionic gonadotropin (0.3 mlU of hormone/tube with B/B0 < 75%), but also bound β and α subunits of human chorionic gonadotropin, 12 and 800 folds lower than human chorionic gonadotropin respectively No binding was observed with carboxy terminal peptides of β-human chorionic gonadotropin ranging from 93 to 145 amino acid residues, indicating the lack of recognition of the C-terminal region. No cross-reaction with human leutinizing hormone was obtained at the physiological surge levels, a significant competition (B/B0 < 75 %. obtainable only at 60 mlU of LER 960 human leutinizing hormone/ tube. The antibody had heavy chain of IgG1 and light chain of kappa type. It neutralized the bio-activity of human chorionic gonadotropin bothin vitro and invivo.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Human Chorionic Gonadotropin; Monoclonal Anti-hCG Antibody; Radioimmuno-assay; Biological Neutralization
ID Code:50532
Deposited On:26 Jul 2011 08:05
Last Modified:18 May 2016 04:47

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