Cloning of Y derived DNA sequences of bovine origin in Escherichia coli

Khandekar, Pramod ; Talwar, G. P. ; Chaudhury, Renu (1986) Cloning of Y derived DNA sequences of bovine origin in Escherichia coli Journal of Biosciences, 10 (4). pp. 481-486. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/10/481-486.pdf

Related URL: http://dx.doi.org/10.1007/BF02900509

Abstract

We have constructed a partial library of Y chromosome derived DNA sequences of bovine origin inEscherichia coli. That, the recombinants arc Y derived and Y specific was ascertained by differential colony hybridization using male and female DNA probes. Out of 1000 recombinants analysed, 17 were found to be Y derived as well as Y specific and were of repetitive nature. Restriction analysis revealed that most of them had short DNA inserts.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Cloning; Repetitive DNA; Bovine Y Chromosome; Hybridization
ID Code:50528
Deposited On:26 Jul 2011 08:09
Last Modified:18 May 2016 04:47

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