Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate

Rao, B. N. Narasinga ; Ramakrishnan, C. ; Balaram, P. (1979) Metal binding to pyridoxal derivatives. An NMR study of the interaction of Eu(III) with pyridoxal phosphate and pyridoxamine phosphate Journal of Biosciences, 1 (1). pp. 35-47. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/1/35-47.pdf

Related URL: http://dx.doi.org/10.1007/BF02702885

Abstract

The solution conformations of pyridoxal-5′-phosphate and pyridoxamine-5′-phosphate have been investigated using Eu(III) as a nuclear magnetic resonance shift probe. Binding of Eu(III) to pyridoxal phosphate results in the formation of two complexes, at the phosphate group and theo-hydroxy-aldehyde moiety, which are in slow exchange on the nuclear magnetic resonance time-scale. The lanthanide-induced pseudo contact shifts calculated using the McConnell-Robertson equation (J. Chem. Soc. (1950), 22, 1561) are in good agreement with the experimentally observed values for both pyridoxal phosphate and pyridoxamine phosphate and lead to a family of closely related conformations.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Conformation; Lanthanide; Nuclear Magnetic Resonance; Pyridoxal-5′ Phosphate; Pyridoxamine-5′-phosphate
ID Code:5051
Deposited On:18 Oct 2010 05:21
Last Modified:16 May 2016 15:37

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