Isolation of an 800 kb contiguous DNA fragment encompassing a 3.5-cM region of chromosome 1 in Arabidopsis using YAC clones

Vijayraghavan, Usha ; Siddiqi, Imran ; Meyerowitz, Elliot (1995) Isolation of an 800 kb contiguous DNA fragment encompassing a 3.5-cM region of chromosome 1 in Arabidopsis using YAC clones Genome, 38 (4). pp. 817-823. ISSN 0831-2796

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Official URL: http://www.nrcresearchpress.com/doi/abs/10.1139/g9...

Related URL: http://dx.doi.org/10.1139/g95-105

Abstract

The apetala1 mutation of Arabidopsis affects floral meristem identity and the development of sepal and petal primordia of the flower. We mapped the available RFLP markers on chromosome 1 that are in the general vicinity of apetala1 on a fine structure map and then chose the closest RFLP as a starting point for contiguous DNA (contig) generation. We report here a contig of about 800 kilobases (kb) that spans a 3.5 cM region of chromosome 1. We used genomic libraries of Arabidopsis prepared in yeast artificial chromosome (YAC) vectors and the detailed characterization of 19 YACs is reported. RFLPs displayed by the end fragments from the walk were mapped to align and correlate the genetic and physical maps for this region of chromosome 1. In this segment of the genome, 1 cM corresponds to a little over 200 kb of physical distance.

Item Type:Article
Source:Copyright of this article belongs to NRC Research Press.
Keywords:Arabidopsis; Apetala1; Chromosome Walking
ID Code:50412
Deposited On:23 Jul 2011 10:51
Last Modified:09 Oct 2011 06:07

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