Uptake and degradation of cyclic AMP by chloronema cell

Sharma, Shobhona ; Johri, Man Mohan (1982) Uptake and degradation of cyclic AMP by chloronema cell Plant Physiology, 69 (6). pp. 1401-1403. ISSN 0032-0889

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Official URL: http://www.plantphysiol.org/content/69/6/1401.abst...

Related URL: http://dx.doi.org/10.1104/pp.69.6.1401


Suspension cultures of intact chloronema cells of the moss Funaria hygrometrica take up [3H]cAMP and degrade it rapidly. The increase in total radioactivity accumulated by the cells was linear up to 30 minutes. Initially, the major degradation products were 5'-AMP and adenosine, but later predominantly ADP and ATP. In spite of rapid degradation, the amount of extracellularly applied cAMP retained by the cells is about 4- fold higher than the maximum endogenous level of cAMP reported previously (Handa, Johri 1977 Plant Physiol 59: 490-4%). The uptake showed a distinct dependence on the density of the culture. Cells at a lower cell density (1-2 milligrams per milliliter) accumulated 4 to 6 times more radioactivity than the cells at high density (gt;10 milligrams per milliliter). The cyclic nucleotide phosphodiesterase (cNPDE) activity of whole cells (18 milliunits per milligram protein) was comparable to that of protoplasts (23 milliunits per milligram protein), but about 4-fold lower than that of lysed protoplasts (80 milliunits per milligram protein), indicating an intracellular degradation of cAMP by chloronema cells.

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