Functional complementation of yeast ribosomal P0 protein with Plasmodium falciparum P0

Aruna, K. ; Chakraborty, Tirtha ; Rao, Pavitra N. ; Santos, Cruz ; Ballesta, Juan P. G. ; Sharma, Shobhona (2005) Functional complementation of yeast ribosomal P0 protein with Plasmodium falciparum P0 Gene, 357 (1). pp. 9-17. ISSN 0378-1119

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A complex of three phosphoproteins (P0, P1 and P2) constitutes the stalk region at the GTPase center of the eukaryotic large ribosomal subunit, amongst which the protein P0 plays the most crucial role. Earlier studies have shown the functional complementation of the conditional P0-null mutant of Saccharomyces cerevisiae (W303dGP0) with orthologous P0 genes from fungal and mammalian organisms, but not the protozoan parasite Leishmania infantum. In this paper we show that the PfP0 gene from the protozoan malaria parasite Plasmodium falciparum can functionally complement the conditional P0-null W303dGP0 mutant of S. cerevisiae. Unlike the above orthologous genes, PfP0 gene could also rescue the D67dGP0 strain, which in addition to being a conditional null for ScP0 gene, is a null-mutant for both ScP1α and β genes. However, under stress conditions such as high temperature, salt and osmolarity, PfP0 gene could not rescue D67dGP0 strain. Ribosomes purified from W303dGP0 carrying PfP0 gene did not contain ScP1 protein, indicating a lack of binding of ScP1 to PfP0 protein. Yeast 2-hybrid analysis further confirmed the lack of binding of ScP1 to PfP0 protein. The polymerizing activities of ribosomes with ScP0 or PfP0 protein, in the absence of ScP1 protein, were found to be about 40-45% that of ribosomes with all the yeast P-proteins. In its sensitivity to the inhibitor sordarin, PfP0 was similar to the P0 protein from the fungus Aspergillus fumigatus. These results indicate a closer functional relationship of P. falciparum P0 gene to fungal P0 genes.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Malaria Parasite; Acidic Proteins; Sordarin; Orthologous P0; Stress Conditions
ID Code:50362
Deposited On:23 Jul 2011 09:17
Last Modified:23 Jul 2011 09:17

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