Enzyme activities associated with developing wheat (Triticum aestivum L.) grain amyloplasts

Mahajan, R. ; Singh, Randhir (1990) Enzyme activities associated with developing wheat (Triticum aestivum L.) grain amyloplasts Journal of Biosciences, 15 (2). pp. 77-82. ISSN 0250-5991

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Official URL: http://www.ias.ac.in/jarch/jbiosci/15/77-82.pdf

Related URL: http://dx.doi.org/10.1007/BF02703371


Intact amyloplasts from endosperm of developing wheat grains have been isolated by first preparing the protoplasts and then fractionating the lysate of the protoplasts on percoll and ficoll gradients, respectively. Amyloplasts isolated as above were functional and not contaminated by cytosol or by organelles likely to be involved in carbohydrate metabolism. The enzyme distribution studies indicated that ADP-glucose pyrophosphorylase and starch synthase were confined to amyloplasts, whereas invertase, sucrose synthase, UDP-glucose pyrophosphorylase, hexokinase, phosphofructokinase-2 and fructose-2,6-P2ase were absent fro the amyloplast and mainly confined to the cytosol. Triose-P isomerase, glyceraldehyde-3-P dehydrogenase, phosphohexose isomerase, phosphoglucomutase, phosphofructokinase, aldolase, PPi-fructose-6-P-1 phosphotransferase, and fructose-l,6-P2ase, though predominantly cytosolic, were also present in the amyloplast. Based on distribution of enzymes, a probable pathway for starch biosynthesis in amyloplasts of developing wheat grains has been proposed.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:Wheat; Triticum aestivum; Amyloplasts; Enzymes; Carbohydrate Metabolism
ID Code:50091
Deposited On:21 Jul 2011 14:43
Last Modified:18 May 2016 04:30

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