Products of photosynthetic 14CO2 fixation and related enzyme activities in fruiting structures of chickpea

Singal, H. R. ; Sheoran, I. S. ; Singh, Randhir (1986) Products of photosynthetic 14CO2 fixation and related enzyme activities in fruiting structures of chickpea Physiologia Plantarum, 66 (3). pp. 457-462. ISSN 0031-9317

Full text not available from this repository.

Official URL:

Related URL:


Fruiting structures of a number of legumes including chickpea are known to carry out photosynthetic CO2 assimilation, but the pathway of CO2 fixation and particularly the role of phosphoenolpyruvate carboxylase (EC in these tissues is not clear. Activities of some key enzymes of the Calvin cycle and C4 metabolism, rates of 14CO2 fixation in light and dark, and initial products of photosynthetic 14CO2 fixation were determined in podwall and seedcoat (fruiting structures) and their subtending leaf in chickpea (Cicer arietinum L.). Compared to activities of ribulose-1,5-bisphosphate carboxylase (EC and other Calvin cycle enzyme, viz. NADP+-glyceraldehyde-3-phosphate dehydrogenase (EC, NAD+-glyceraldehyde-3-phosphate dehydrogenase (EC and ribulose-5-phosphate kinase (EC, the levels of phosphoenolpyruvate carboxylase and other enzymes of C4 metabolism viz. NADP+-malate dehydrogenase (EC, NAD+-malate dehydrogenase (EC, NADP+ malic enzyme (EC, NAD+-malic enzyme (EC, glutamate oxaloacetate transaminase (EC and glutamate pyruvate transaminase (EC, were generally much higher in podwall and seedcoat than in the leaf. Podwall and seedcoat fixed 14CO2 in light and dark at much higher rates than the leaf. Short-term assimilation of 14CO2 by illuminated fruiting structures produced malate as the major labelled product with less labelling in 3-phosphoglycerate, whereas the leaf showed a major incorporation into 3-phosphoglycerate. It seems likely that the fruiting structures of chickpea utilize phosphoenolpyruvate carboxylase for recapturing the respired carbon dioxide.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:CO2 Fixation; PEP Carboxylase; Podwall; Seedcoat
ID Code:50075
Deposited On:21 Jul 2011 14:40
Last Modified:21 Jul 2011 14:40

Repository Staff Only: item control page