Sopory, Sudhir K. ; Jacobsen, Evert ; Wenzel, Gerhard (1978) Production of monohaploid embryoids and plantlets in cultured anthers of Solanum tuberosum Plant Science Letters, 12 (1). pp. 47-54. ISSN 0304-4211
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0304-4211(78)90038-X
Abstract
The production of monohapoid Solanum tubersum embryoids and plantlets with 2n=12 chromosomes from microspores cultured within anthers, is reported. By selecting the precise developmental stage of microspores and by modifying the culture media, it was possible to achieve microspore embryogenesis in 35% of the plated anthers. Potato extract was found to be beneficial, but the response could be obtained even in Murashige and Skoog [1] medium. For maximum response, presence of 6% sucrose, 0.5% activated charcoal and 4×10−6 M 6-benzylaminopurine (BAP) was necessary. The cytological examination of young embryoids and of root tips of developed plants revealed monohaploids as well as dihaploids.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 49907 |
| Deposited On: | 21 Jul 2011 09:09 |
| Last Modified: | 21 Jul 2011 09:09 |
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