Enzyme-linked immunolysis assay for tumour-specific surface antigens

Khar, Ashok ; Sitaramam, V. (1986) Enzyme-linked immunolysis assay for tumour-specific surface antigens Journal of Biochemical and Biophysical Methods, 13 (3). pp. 161-169. ISSN 0165-022X

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Official URL: http://www.sciencedirect.com/science/article/pii/0...

Related URL: http://dx.doi.org/10.1016/0165-022X(86)90089-8


Expression of the activity of the cytosolic enzyme lactate dehydrogenase released on cell lysis by tumour specific antibodies in the presence of complement was investigated by direct assay of the expression of the occluded enzyme tumour cell suspensions. This method, called Enzyme Linked Immunolysis assay (ELILA) was shown to be more sensitive than conventional cytotoxic assays. The data fit well in the linear regression model, so that the technique can be used for quantitation of the antibody titres.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Histiocytic Cell Line; Cytotoxic Antibodies; Immunolysis; Linear Regression
ID Code:49845
Deposited On:21 Jul 2011 10:04
Last Modified:21 Jul 2011 10:04

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