A novel zinc bis(thiosemicarbazone) complex for live cell imaging

Dayal, Disha ; Palanimuthu, Duraippandi ; Shinde, Sridevi Vijay ; Somasundaram, Kumaravel ; Samuelson, Ashoka G. (2011) A novel zinc bis(thiosemicarbazone) complex for live cell imaging Journal of Biological Inorganic Chemistry, 16 (4). pp. 621-632. ISSN 0949-8257

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Official URL: http://www.springerlink.com/content/3867l336636558...

Related URL: http://dx.doi.org/10.1007/s00775-011-0764-0

Abstract

Fluorescent zinc complexes have recently attracted a lot of interest owing to their vast applications in cellular imaging. We report the synthesis as well as physical, chemical and biological studies of a novel zinc glyoxalbis(4-methyl-4-phenyl-3-thiosemicarbazone), [Zn(GTSC)]3, complex. As compared with the well-studied zinc biacetylbis(4-methyl-3-thiosemicarbazone), Zn(ATSM), complex, which was used as a reference, [Zn(GTSC)]3 had 2.5-fold higher fluorescence. When cellular fluorescence was measured using flow cytometry, we observed that [Zn(GTSC)]3 had 3.4-fold to 12-fold higher fluorescence than Zn(ATSM) in various cell lines (n=9) of different tissue origin. Confocal fluorescence microscopy results showed that [Zn(GTSC)]3 appeared to have a nuclear localization within 30 min of addition to MCF7 cells. Moreover, [Zn(GTSC)]3 showed minimal cytotoxicity compared with Zn(ATSM), suggesting that [Zn(GTSC)]3 may be less deleterious to cells when used as an imaging agent. Our data suggest that the novel [Zn(GTSC)]3 complex can potentially serve as a biocompatible fluorescent imaging agent for live cells.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Bis(Thiosemicarbazone); Imaging Agent; Cell Viability; Breast Cancer; Zinc
ID Code:49681
Deposited On:20 Jul 2011 14:00
Last Modified:20 Jul 2011 14:00

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