Actin cytoskeleton as a putative target of the neem limonoid azadirachtin A

Anuradha, Aritakula ; Annadurai, Ramaswamy S. ; Shashidhara, L. S. (2007) Actin cytoskeleton as a putative target of the neem limonoid azadirachtin A Insect Biochemistry and Molecular Biology, 37 (6). pp. 627-634. ISSN 0965-1748

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Limonoids isolated from the Indian neem tree (Azadirachta indica) have been gaining global acceptance in agricultural applications and in contemporary medicine for their myriad but discrete properties. However, their mode of action is still not very well understood. We have studied the mode of action of Azadirachtin A, the major limonoid of neem seed extracts, using Drosophila melanogaster as the model system. Azadirachtin A induces moderate-to-severe phenotypes in different tissues in a dose-dependent manner. At the cellular level, Azadirachtin A induces depolymerization of Actin leading to arrest of cells and subsequently apoptosis in a caspase-independent manner. Azadirachtin A-induced phenotypes were rescued by the over-expression of Cyclin E in a tissue-dependent manner. Cyclin E, which caused global rescue of Azadirachtin A-induced phenotypes, also effected rearrangement of the actin filaments. These results suggest that probably actin is a target of Azadirachtin A activity.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:β-tubulin; Cyclin E; Limonoid; Apoptosis; Biopesticide
ID Code:49640
Deposited On:20 Jul 2011 14:13
Last Modified:20 Jul 2011 14:13

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