Drosophila twins regulates Armadillo levels in response to Wg/Wnt signal

Bajpai, Ruchi ; Makhijani, Kalpana ; Ramesh Rao, Prashanth ; Shashidhara, L. S. (2004) Drosophila twins regulates Armadillo levels in response to Wg/Wnt signal Development, 131 . pp. 1007-1016. ISSN 0950-1991

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Official URL: http://dev.biologists.org/content/131/5/1007.short

Related URL: http://dx.doi.org/10.1242/dev.00980


Protein Phosphatase 2A (PP2A) has a heterotrimeric-subunit structure, consisting of a core dimer of ∼36 kDa catalytic and ∼65 kDa scaffold subunits complexed to a third variable regulatory subunit. Several studies have implicated PP2A in Wg/Wnt signaling. However, reports on the precise nature of PP2A role in Wg/Wnt pathway in different organisms are conflicting. We show that twins (tws), which codes for the B/PR55 regulatory subunit of PP2A in Drosophila, is a positive regulator of Wg/Wnt signaling. In tws-wing discs both short-and long-range targets of Wingless morphogen are downregulated. Analyses of tws-mitotic clones suggest that requirement of Tws in Wingless pathway is cell-autonomous. Epistatic genetic studies indicate that Tws functions downstream of Dishevelled and upstream of Sgg and Armadillo. Our results suggest that Tws is required for the stabilization of Armadillo/β-catenin in response to Wg/Wnt signaling. Interestingly, overexpression of, otherwise normal, Tws protein induce dominant-negative phenotypes. The conflicting reports on the role of PP2A in Wg/Wnt signaling could be due to the dominant-negative effect caused by the overexpression of one of the subunits.

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