Restriction fragment length polymorphism and random amplified polymorphic DNA analysis of chickpea accessions

Banerjee, H. ; Pai, R. A. ; Sharma, R. P. (1999) Restriction fragment length polymorphism and random amplified polymorphic DNA analysis of chickpea accessions Biologia Plantarum, 42 (2). pp. 197-208. ISSN 0006-3134

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Official URL: http://www.springerlink.com/content/n6710728002708...

Related URL: http://dx.doi.org/10.1023/A:1002152416883

Abstract

Genetic diversity analysis was carried out in chickpea accessions using restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. RFLP analysis using 26 Pst I sub-genomic clones on ten chickpea accessions in 130 probe-enzyme combinations detected polymorphism with only two clones. Pst I clones, CG 141 detected polymorphism in ICC 4918 and Pusa 209 while CG 500 detected polymorphism in Pusa 261, ILC 26 and in ILC 13326. These clones detected very few polymorphic markers. Analysis using 10 Eco RI clones on twelve chickpea accessions have shown better hybridisation signal and one clone detected polymorphism in Pusa 256. RFLP analysis of both cultivated and wild Cicer species using heterologous DNA probe Cab3C revealed polymorphism only in wild Cicer species (Cicer reticulatum L., JM 2100). RAPD analysis of 13 chickpea accessions which includes mutants of C 235 and E100Y showed greater degree of polymorphism with 1-5 unique DNA bands for all the accessions. Phylogenetic analysis of the RAPD data helped to group the accessions. C 235 and its mutants were found to be closely grouped while E100Y and its mutant E100Ym grouped apart. Desi and kabuli chickpea accessions however, could not be separately grouped.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Cicer arietinum; Cicer reticulatum; DNA Polymorphism; Genetic Diversity; Phylogeny
ID Code:49374
Deposited On:20 Jul 2011 05:54
Last Modified:20 Jul 2011 05:54

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