Rinderpest virus RNA polymerase subunits: mapping of mutual interacting domains on the large protein L and phosphoprotein P

Chattopadhyay, Anasuya ; Shaila, M. S. (2004) Rinderpest virus RNA polymerase subunits: mapping of mutual interacting domains on the large protein L and phosphoprotein P Virus Genes, 28 (2). pp. 169-178. ISSN 0920-8569

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Official URL: http://www.springerlink.com/content/t04p950350154j...

Related URL: http://dx.doi.org/10.1023/B:VIRU.0000016855.25662.95

Abstract

The RNA dependant RNA polymerase of negative sense RNA viruses is composed of two subunits-the Large protein (L) and the Phosphoprotein (P). These two proteins have to form a complex in order to carry out genome transcription and replication. Employing the baculovirus expression system, we demonstrate here, the specific in vivo interaction between the L and P proteins of Rinderpest virus and also the stabilization of L protein when it is present as L+P complex. The regions on either protein involved in such interaction has been studied using the yeast two-hybrid system which indicates that the P binding region resides within the amino terminal 380 amino acid residues of L protein. The L binding region on P protein has been mapped to lie within 347-490 amino acids.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Interaction; Large Protein; Phosphoprotein; Rinderpest Virus
ID Code:49345
Deposited On:20 Jul 2011 06:17
Last Modified:20 Jul 2011 06:17

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