Interface peptide of Alzheimer's amyloid beta: application in purification

Das, Utpal ; Hariprasad, Gururao ; Pasha, Santosh ; Mann, Anita ; Ganguli, Munia ; Sharma, Sujata ; Kaur, Punit ; Singh, Tej P. ; Srinivasan, Alagiri (2007) Interface peptide of Alzheimer's amyloid beta: application in purification Biochemical and Biophysical Research Communications, 362 (2). pp. 538-542. ISSN 0006-291X

Full text not available from this repository.

Official URL:

Related URL:


Protein precipitation is a process commonly observed during bacterial expression of heterologous proteins. The high concentration of currently used solubilizing agents limits the scope of purification procedures. Protein solubilizers acting at very low concentrations will allow function-based purification protocols. Such applications are possible where sequence dependent protein-protein interactions occur. Oligomerization and higher order structure formation by proteins are examples where the interface peptide sequences are inhibitors of such interactions. In this study, we show that a partial sequence of Alzheimer's amyloid beta (Aβ) peptide can inhibit the aggregation. This enables the purification of protein containing Aβ sequence.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Alzheimer's Amyloid Beta Peptide; Aggregation Inhibition; Purification; Suppression Of Cytotoxicity
ID Code:49155
Deposited On:19 Jul 2011 04:35
Last Modified:19 Jul 2011 04:35

Repository Staff Only: item control page