Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility

Kumar, Vijay ; Hassan, Md. Imtaiyaz ; Tomar, Anil Kumar ; Kashav, Tara ; Nautiyal, Jaya ; Singh, Sarman ; Singh, Tej P. ; Yadav, Savita (2009) Proteomic analysis of heparin-binding proteins from human seminal plasma: a step towards identification of molecular markers of male fertility Journal of Biosciences, 34 (6). pp. 899-908. ISSN 0250-5991

[img]
Preview
PDF - Publisher Version
717kB

Official URL: http://www.ias.ac.in/jbiosci/dec2009/899.pdf

Related URL: http://dx.doi.org/10.1007/s12038-009-0104-5

Abstract

Glycosaminoglycans, especially heparin, are involved in various cell processes such as apoptosis, cell cycle control, platelet activation, capacitation, acrosome reaction and sperm decondensation. Heparin-binding proteins (HBPs) are essential constituents of human seminal fluid, which bind to sperm lipids containing the phosphorylcholine group and mediate the fertilization process. We utilized a proteomic set-up consisting of affinity chromatography, isoelectric focusing (IEF) coupled with matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (MALDI TOF/MS) for protein analysis of human HBPs. We resolved 70 different spots on two-dimensional (2-D) gel and subsequently identified these proteins. Forty different types of proteins were identified. Functional analysis revealed that 38% of the proteins belonged to the enzyme category, 20% were involved in RNA processing and transcription, 18% in structure and transport function, and 16% in cell recognition and signal transduction. We also identified 8% of proteins with unknown functions, although their expression in seminal fluid has been documented. Proteins of seminal fluid that bind heparin may be directly involved in sperm capacitation and acrosome reaction (AR), which are the two critical steps for fertilization. This information on HBPs would be useful for identifying potential biomarkers of fertility in the near future.

Item Type:Article
Source:Copyright of this article belongs to Indian Academy of Sciences.
Keywords:2D Gel Electrophoresis; Human Seminal Plasma; Heparin-Binding Proteins; MALDI-TOF; Sperm Capacitation
ID Code:49153
Deposited On:19 Jul 2011 04:35
Last Modified:18 May 2016 03:58

Repository Staff Only: item control page