Production of Eruca-Brassica hybrids by embryo rescue

Agnihotri, Abha ; Gupta, Vibha ; Lakshmikumaran, Malathi S. ; Shivanna, K. R. ; Prakash, Shyam ; Jagannathan, V. (1990) Production of Eruca-Brassica hybrids by embryo rescue Plant Breeding, 104 (4). pp. 281-289. ISSN 0179-9541

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Allied species of crop Brassicas have potential value as donors of useful nuclear/organelle genomes. Eruca sativa, a member of subtribe Brassicineae, is resistant to white rust and well adapted to drought. Attempts to hybridize it with Brassica campestris by conventional methods were unsuccessful. However, hybrids were obtained by embryo rescue and the hybrid embryos were found to produce numerous secondary embryos in upto 7 to 8 subcultures. Plantlets developed from them exhibited morphological characteristics of both parents. The chromosome number of 2n=42 showed that they were amphidiploids. The plants were allotctraploids (2n=42) and showed 21 bivalents at M1 of meiosis. Hybridization of total DNA of the hybrids with two probes, a B. campestris tandem repeat DNA and 18s ribosomal DNA of wheat showed that it was derived from the genome of both parents. The hybrids are self-fertile and show the same high fertility even in A3 generation. They were selfed or crossed with B. juncea, B. campestris and B. nigra to screen for useful agronomical traits. Six more embryos of E. saliva × B. campestris have been obtained and their growth is being studied. Preliminary small scale field trials indicate that the selfed hybrid is comparable in yield with a high yielding commercial variety of B. juncea.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
Keywords:Eruca sativa; Brassica campestris; Interspecific Hybrids; Embryo Rescue; Secondary Embryos; Amphidiploid; DNA Analysis
ID Code:49046
Deposited On:18 Jul 2011 12:52
Last Modified:11 Jul 2012 04:49

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