Binding constant measurement by hyper-Rayleigh scattering: bilirubin-human serum albumin binding as a case study

Ranjini, Arumugam Sri ; Das, Puspendu K. ; Balaram, Padmanabhan (2005) Binding constant measurement by hyper-Rayleigh scattering: bilirubin-human serum albumin binding as a case study Journal of Physical Chemistry B, 109 (12). pp. 5950-5953. ISSN 1089-5647

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Official URL: http://pubs.acs.org/doi/abs/10.1021/jp047268n

Related URL: http://dx.doi.org/10.1021/jp047268n

Abstract

In this paper, a new application of the hyper-Rayleigh scattering technique in determining multiple binding constants of a small molecule like bilirubin to a macromolecule like the protein human serum albumin has been demonstrated. Human serum albumin has two binding sites for bilirubin, and the binding constants have been measured by carrying out a second harmonic titration of the protein against bilirubin and vice versa. The measured binding constants K1 = 1.5 ± 0.43 × 107 M-1 and K2 = 1.01 ± 0.16 × 106 M-1 agree well with the reported values obtained by other methods.

Item Type:Article
Source:Copyright of this article belongs to American Chemical Society.
ID Code:4865
Deposited On:18 Oct 2010 06:26
Last Modified:11 May 2012 11:21

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