Krishnan, K. S. ; Balaram, P. (1976) Mammalian brain plasma membranes: isolation, enzymatic, and chemical characterization Experimental Cell Research, 101 (2). pp. 299-306. ISSN 0014-4827
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Official URL: http://linkinghub.elsevier.com/retrieve/pii/001448...
Related URL: http://dx.doi.org/10.1016/0014-4827(76)90381-5
Abstract
Two variants of a simplified procedure for the isolation of plasma membrane fractions from monkey and rat brains, are described. The preparations show marked enrichments in the marker enzymes, (Na+-K+) adenosine triphosphatase, acetylcholinesterase, 5′-nucleotidase and adenylate cyclase. Lipid analysis and a protein electrophoretic pattern are presented. An enzymatic check has been made to assess for contamination by other cellular organelles. The amino acid composition of brain membrane proteins show a resemblance to the reported composition of erythrocyte ghost proteins but differ from myelin proteins.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| ID Code: | 4846 |
| Deposited On: | 18 Oct 2010 06:31 |
| Last Modified: | 16 May 2011 09:37 |
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