Rodrigues, Veronica ; Bülthoff, Isabelle (1985) Freeze-substitution of Drosophila heads for subsequent [3H]2-deoxyglucose autoradiography Journal of Neuroscience Methods, 13 (3-4). pp. 183-190. ISSN 0165-0270
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Official URL: http://www.sciencedirect.com/science/article/pii/0...
Related URL: http://dx.doi.org/10.1016/0165-0270(85)90066-4
Abstract
High resolution of [3H]2-deoxyglucose labelling was obtained in autoradiographs of Drosophila brains after freeze-substitution in anhydrous acetone at ≄76°C. This method was applied to preparations which received visual, olfactory and mechanosensory stimulation. The autoradiographs were compared to those obtained after freeze-drying. Freeze-substitution, which has proved to be technically simple, rapid and inexpensive, yields a good quality of tissue preservation and hence is recommended for tissue dehydration prior to autoradiography.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Elsevier Science. |
| Keywords: | Freeze-substitution; Dehydration; Deoxyglucose; Drosophila; Visual; Olfactory |
| ID Code: | 48372 |
| Deposited On: | 14 Jul 2011 06:35 |
| Last Modified: | 14 Jul 2011 06:35 |
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