Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films

Gole, Anand ; Dixit, Vaishali ; Lala, Neeta ; Sainkar, S. R. ; Pant, Aditi ; Sastry, Murali (2002) Patterned assembly of Yarrowia lipolytica yeast cells onto thermally evaporated octadecylamine films Colloids and Surfaces B: Biointerfaces, 25 (4). pp. 363-368. ISSN 0927-7765

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Official URL: http://www.sciencedirect.com/science/article/pii/S...

Related URL: http://dx.doi.org/10.1016/S0927-7765(02)00005-X

Abstract

Thermally evaporated patterned films of the lipid, octadecylamine (ODA), have been used in the immobilization of the hydrocarbon-degrading cells, Yarrowia lipolytica. The immobilization of the yeast cells occurs on hydrophobic surfaces presented by the lipid film elements in the patterned structure, the attachment of the cells to the lipid film occurring possibly through hydrophobic interactions between the hydrocarbon chains of the fatty amine film and the cell wall of the yeasts. It is observed that the cell immobilization is extremely faithful to the underlying lipid template indicating potential use in tissue engineering as well as materials applications involving specific enzyme-based biotransformations.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Cell Attachment; Immobilization; Lipid Bilayers; Quartz Crystal Microbalance; Hydrophobic Surface
ID Code:47156
Deposited On:06 Jul 2011 14:12
Last Modified:06 Jul 2011 14:12

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