Singh, L. ; Purdom, I. F. ; Jones, K. W. (1977) Effect of different denaturing agents on the detectability of specific DNA sequences of various base compositions by in situ hybridisation Chromosoma, 60 (4). pp. 377-389. ISSN 0009-5915
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Official URL: http://www.springerlink.com/content/j86432743l0031...
Related URL: http://dx.doi.org/10.1007/BF00292860
Abstract
In situ hybridisation of certain AT rich and GC rich satellite DNA complementary RNAs (cRNAs) to their homologous chromosomes at their respective optimal rate temperatures (TOPTS) after denaturation with various reagents (0.2 N HCl, 0.07 N NaOH, 90% formamide and heat) led to the following conclusions. -Heat denaturation of chromosomal DNA in 0.1×SSC at 100°C gives significantly higher grain counts regardless of DNA base composition, HCl denaturation discriminates markedly against GC rich DNA. Chromosome morphology is best preserved after HCl and heat denaturation.
| Item Type: | Article |
|---|---|
| Source: | Copyright of this article belongs to Springer. |
| ID Code: | 46793 |
| Deposited On: | 06 Jul 2011 04:53 |
| Last Modified: | 06 Jul 2011 04:53 |
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