A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola

Upadhyaya, Geetha ; Shivanna, M. B. ; Prakash, H. S. ; Shetty, H. S. (1992) A novel approach to the establishment of dual cultures of pearl millet and Sclerospora graminicola Plant Cell, Tissue and Organ Culture, 31 (3). pp. 203-206. ISSN 0167-6857

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Official URL: http://www.springerlink.com/content/k3j57lt625vk36...

Related URL: http://dx.doi.org/10.1007/BF00036224

Abstract

Dual cultures were successfully established using malformed florets of pearl millet infected with Sclerospora graminicola, the downy mildew pathogen. A higher proportion (86%) of calli from malformed florets formed dual cultures on Murashige and Skoog's (MS) medium with 2 mg l−1 of 2,4-dichlorophenoxy acetic acid (2,4-D), compared to shoot tips (25%). Fungal mycelium covered the entire surface of the callus within 30 days of placement of explants on the MS medium with 2 mg l−1 of 2,4-D. The infected calli also differentiated and produced plantlets when transferred to MS medium without 2,4-D.

Item Type:Article
Source:Copyright of this article belongs to Springer.
Keywords:Dual Culture; Malformed Floret; Pearl Millet; Sclerospora graminicola
ID Code:46737
Deposited On:06 Jul 2011 07:09
Last Modified:06 Jul 2011 07:09

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