Induction of Lipoxygenase in downy mildew resistant seedlings of pearl millet in response to inoculation with Sclerospora graminicola

Babitha, M. P. ; Prakash, H. S. ; Shetty, H. S. (2006) Induction of Lipoxygenase in downy mildew resistant seedlings of pearl millet in response to inoculation with Sclerospora graminicola International Journal of Agriculture and Biology, 8 (4). pp. 560-564. ISSN 1560-8530

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Abstract

Lipoxygenase (LOX) activity was studied in seedlings of pearl millet genotypes resistant and susceptible to downy mildew pathogen Sclerospora graminicola. An increase in LOX activity was observed during the incompatible host-pathogen interaction whereas the activity decreased in compatible ones. Resistant pearl millet seedlings exhibited a 2.4-fold increase in LOX activity after inoculation with the pathogen. The enzyme activity was maximum at 18 h after inoculation. The enzyme activity was maximum in shoot portion of resistant genotype after inoculation. The enzyme activity correlated well with the degree of host-resistance to the pathogen. Substrate-based gel assay revealed five isozymes (LOX-1, LOX-2, LOX-3, LOX-4 & LOX-5) in both susceptible and resistant genotype. An additional isozyme (LOX-6) was unique to only resistant genotype after inoculation.

Item Type:Article
Source:Copyright of this article belongs to Friends Science Publishers.
Keywords:Host-pathogen Interaction; Resistance; Susceptibility; Induction; Defense-related Enzymes; Linoleic Acid
ID Code:46699
Deposited On:06 Jul 2011 07:18
Last Modified:18 May 2016 02:31

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