Nitric oxide donor seed priming enhances defense responses and induces resistance against pearl millet downy mildew disease

Manjunatha, G. ; Niranjan Raj, S. ; Shetty, Nandini P. ; Shekar Shetty, H. (2008) Nitric oxide donor seed priming enhances defense responses and induces resistance against pearl millet downy mildew disease Pesticide Biochemistry and Physiology, 91 (1). pp. 1-11. ISSN 0048-3575

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S00483...

Related URL: http://dx.doi.org/10.1016/j.pestbp.2007.11.012

Abstract

Nitric oxide (NO) donors Nitroso-R-Salt, 2-Nitroso-1-Naphthol and Sodium Nitro Prusside (SNP) were evaluated for their effectiveness in protecting pearl millet [(Pennisetum glaucum L.) R. Br.] plants against downy mildew disease caused by Sclerospora graminicola [(Sacc). Schroet]. Optimization experiments with NO donors showed no adverse effect either on the host or pathogen. Aqueous SNP seed treatment with or without polyethylene glycol (PEG) priming was the most effective in inducing the host resistance against downy mildew both under greenhouse and field conditions. Potassium Ferrocyanide, a structural analog of NO donor lacking NO moiety failed to protect the pearl millet plants from downy mildew indicating a role for NO in induced host resistance. Spatio-temporal studies corroborated that the protection offered by NO donor treatment was systemic in nature and a minimum of 3-day time gap between the inducer treatment and subsequent pathogen inoculation was necessary for maximum resistance development. Disease protection ability of NO donors was also validated as durable in nature. Conversely, prior-treatment with NO scavenger 2-4-carboxyphenyl-4,4,5,5 tetrazoline-1-oxyl-3-oxide potassium salt (C-PTIO) rendered the pearl millet plants relatively susceptible for pathogen infection. Expression of primary defense responses like hypersensitive response, lignin deposition and defense related enzyme phenylalanine ammonialyase -EC 4.3.1.5 (PAL) were enhanced by NO donor treatments.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Nitric Oxide Donors; Pearl Millet; Downy Mildew Disease; Sclerospora graminicola
ID Code:46671
Deposited On:06 Jul 2011 07:20
Last Modified:06 Jul 2011 07:20

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