Isolation and immunochemical characterization of the alcohol-extractable proteins (kafirins) of Sorghum bicolor (L.) Moench

Mazhar, H. ; Chandrashekar, A. ; Shetty, H. S. (1993) Isolation and immunochemical characterization of the alcohol-extractable proteins (kafirins) of Sorghum bicolor (L.) Moench Journal of Cereal Science, 17 (1). pp. 83-93. ISSN 0733-5210

Full text not available from this repository.

Official URL: http://linkinghub.elsevier.com/retrieve/pii/S07335...

Related URL: http://dx.doi.org/10.1006/jcrs.1993.1009

Abstract

The differential extractabilities of kafirins were exploited in purifying α-, β- and γ- kafirins. Polyclonal antibodies were raised against them. Immunoblotting showed no cross reactivity between the three kafirins. Nitrogen analysis, sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and enzyme linked immunosorbent assay (ELISA) were used to demonstrate the qualitative and quantitative differences between kafirins and cross-linked kafirins. ELISA indicated that increased extractability of β- and γ-kafirins was responsible for the increase in the total kafirin extracted when a reducing agent was added to aqueous t-butanol. All three kafirin antibodies reacted with specific prolamins from other tropical millets and cereals suggesting homologies amongst the prolamins contained within them.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
ID Code:46651
Deposited On:05 Jul 2011 12:49
Last Modified:05 Jul 2011 12:49

Repository Staff Only: item control page