Synthesis and fluorescence properties of a probe for membrane anionic sites

Abstract

A convenient synthesis of the fluorescent probe, dansyl cadaverine is reported. The probe binds to erythrocyte membranes and brain lipids with changes in fluorescence spectra but does not interact with bovine serum albumin, trypsin and chymotrypsin. Evidence for its affinity for anionic binding sites is presented. Two classes of binding sites are observed with erythrocyte membranes using tryptophan energy transfer experiments.