Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles

Hema, M. ; Subba Reddy, Ch. V. ; Savithri, H. S. ; Sreenivasulu, P. (2008) Assembly of recombinant coat protein of sugarcane streak mosaic virus into potyvirus-like particles Indian Journal of Experimental Biology, 46 . pp. 793-796. ISSN 0019-5189

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Abstract

Coat protein (CP) gene of sugarcane streak mosaic virus-AP isolate (SCSMV-AP) was expressed in E. coli and recombinant CP (SCSMV-AP rCP) was purified by linear sucrose density gradient centrifugation. Observation of purified SCSMV-AP rCP under electron microscope revealed the presence of potyvirus-like particles (PVLPs). The assembled particles were shown to encapsidate CP gene transcripts by slot-blot hybridization.

Item Type:Article
Source:Copyright of this article belongs to National Institute of Science Communication and Information Resources.
Keywords:Potyvirus-like Particles; Recombinant Coat Protein; Sugarcane Streak Mosaic Virus
ID Code:45981
Deposited On:29 Jun 2011 10:07
Last Modified:29 Jun 2011 10:07

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