Activation of nitrate reductase by calcium and calmodulin

Sane, Prafullachandra V. ; Kumar, Nikhil ; Baijal, Madhulika ; Singh, Kaushal K. ; Kochhar, Vinod K. (1987) Activation of nitrate reductase by calcium and calmodulin Phytochemistry, 26 (5). pp. 1289-1291. ISSN 0031-9422

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Nitrate reductase prepared from the leaves of Amranthus is activated by calcium and a small M, protein factor prepared from spinach by the procedures used for calmodulin preparation. The activation is considerably enhanced if both Ca2+ and the protein factor are present. This activation is inhibited by EGTA, a Ca2+ specific chelator and by anticalmodulin compounds like chlorpromazine. The effect of EGTA is reversed by C2+. The protein factor was identified as calmodulin. The enzyme is also activated by commercially available calmodulin. Calmodulin activation seems to be manifested in the FMNH2-NR moiety of the enzyme molecule.

Item Type:Article
Source:Copyright of this article belongs to Elsevier Science.
Keywords:Nitrate Reductase; Calcium; Calmodulin
ID Code:45136
Deposited On:25 Jun 2011 05:50
Last Modified:25 Jun 2011 05:50

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