Distribution of variable fluorescence among subchloroplast fractions

Park, R. B. ; Steinback, K. E. ; Sane, P. V. (1971) Distribution of variable fluorescence among subchloroplast fractions Biochimica et Biophysica Acta (BBA) - Bioenergetics, 253 (1). pp. 204-207. ISSN 0005-2728

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Related URL: http://dx.doi.org/10.1016/0005-2728(71)90245-3


Spinach chloroplasts were fragmented by the French press technique and were separated by fractional centrifugation. Variable fluorescence was assayed by adapting a Cary 14 spectrophotometer for use as a modulated detector. The amount of variable fluorescence was directly related to the abundance of Photosystem II in the fractions. The 160K fraction which possesses negligible Photosystem II activity also possesses negligible variable fluorescence. The yield of background fluorescence from the 160K fraction is also much less than that from the other fractions. The presence of inactive chlorophyll in the 160K fraction cannot be explained by damage of Photosystem II during French press fragmentation.

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