Preparation and properties of immobilized amyloglucosidase

Nithianandam, V. S. ; Srinivasan, K. S. V. ; Thomas Joseph, K. ; Santappa, M. (1981) Preparation and properties of immobilized amyloglucosidase Biotechnology and Bioengineering, 23 (10). pp. 2273-2282. ISSN 0006-3592

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Amyloglucosidase was immobilized on a copolymer of methyl methacrylate and 2-dimethylaminoethyl methacrylate. The resulting immobilized amyloglucosidase has 19% of the soluble enzyme specific activity. The pH optimum of immobilized amyloglucosidase is shifted towards acidity by 1.9 units. The temperature optimum of immobilized enzyme is shifted upward by 5°C. The immobilized amyloglucosidase has the maximum stability at pH 4.6, whereas the soluble enzyme has maximum stability at pH 5.5. While soluble amyloglucosidase has a maximum thermal stability at 50°C, the stability of the immobilized amyloglucosidase steadily decreases with the increase in temperature.

Item Type:Article
Source:Copyright of this article belongs to John Wiley and Sons.
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Deposited On:11 Jun 2011 06:28
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