Regulation of protein synthesis in the first trimester human placenta by 17β-oestradiol and progesterone

Chidananda Sharma, S. ; Kumari, Usha ; Dighe, Rajan R. ; Jagannadha Rao, A. (1990) Regulation of protein synthesis in the first trimester human placenta by 17β-oestradiol and progesterone Placenta, 11 (1). pp. 63-74. ISSN 0143-4004

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Official URL: http://linkinghub.elsevier.com/retrieve/pii/S01434...

Related URL: http://dx.doi.org/10.1016/S0143-4004(05)80444-8

Abstract

Addition of 17β-oestradiol or progesterone to first trimester human placental explants in vitro resulted in the stimulation of protein synthesis, as seen by autofluorographic analysis of placental tissue and medium proteins. An increase in the incorporation of [35S]methionine into trichloroacetic acid-precipitable proteins was seen, following the addition of 17β-oestradiol. Use of aromatase inhibitor to block the synthesis of 17β-oestradiol inhibited the protein synthesis and while addition of cyclohexamide blocked both basal- and 17β-oestradiol-induced protein synthesis, actinomycin-D blocked only 17β-oestradiol induced protein synthesis. Double labelling of placental proteins in the presence and absence of 17β-oestradiol also indicated that there is a significant stimulation of protein synthesis by 17β-oestradiol. Based on these results it is suggested that oestradiol has a role in regulation of placental protein synthesis.

Item Type:Article
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ID Code:43036
Deposited On:09 Jun 2011 09:14
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